Coding

Part:BBa_K4649004:Design

Designed by: Sophia Eowyn Hsu   Group: iGEM23_EastCoastBioCrew   (2023-09-09)


pstB codon optimized for Rhizobium tropici


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 694
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 694
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 694
    Illegal NgoMIV site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Rhizobium tropici


Source

Escherichia coli strain K12 genome (UniProt accession: P0AAH0)

References